منابع مشابه
FluoroGold-Labeled Organotypic Retinal Explant Culture for Neurotoxicity Screening Studies
Preclinical toxicity screening of the new retinal compounds is an absolute requirement in the pathway of further drug development. Since retinal neuron cultivation and in vivo studies are relatively expensive and time consuming, we aimed to create a fast and reproducible ex vivo system for retinal toxicity screening. For this purpose, we used rat retinal explant culture that was retrogradely la...
متن کاملTime-Lapse Retinal Ganglion Cell Dendritic Field Degeneration Imaged in Organotypic Retinal Explant Culture
PURPOSE To develop an ex vivo organotypic retinal explant culture system suitable for multiple time-point imaging of retinal ganglion cell (RGC) dendritic arbors over a period of 1 week, and capable of detecting dendrite neuroprotection conferred by experimental treatments. METHODS Thy1-YFP mouse retinas were explanted and maintained in organotypic culture. Retinal ganglion cell dendritic arb...
متن کاملNucleus pulposus explant culture model.
Many of the therapeutic interventions for intervertebral disc degeneration attempt to repopulate the nucleus pulposus (NP) tissue; however, NP cells are heterogeneous and not well characterized. To address this, we have investigated the morphology, extracellular gene and protein expression, and apoptosis changes in NP explants cultured in vitro with or without chondrogenic reagents for differen...
متن کاملHypothermia protects retinal cells against cobalt-chloride induced hypoxic damage in a porcine organ explant culture
Purpose: Cobalt-chloride (CoCl2) induces a strong degeneration of the porcine retina through hypoxic mechanisms. Neurons of the inner retina layers are particularly affected. This model is used to study possible protective effects of hypothermia treatment. Methods: Organotypic cultures of porcine retinas were cultured and treated with 300 μM CoCl2 for 48 h. Hypothermia (30°C) was applied for th...
متن کاملM-opsin protein degradation is inhibited by MG-132 in Rpe65−/− retinal explant culture
PURPOSE The 65 kDa retinal pigment epithelium-specific protein, RPE65, is an essential enzyme for 11-cis-retinal synthesis in the eye. Mutations of the RPE65 gene in humans result in severe vision loss, and Rpe65(-/-) mice show early cone photoreceptor degeneration. We used an explant culture system to evaluate whether posttranslational downregulation of M-opsin protein in Rpe65(-/-) mice is ca...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1032